Monoclonal Antibody to Alanine Aminotransferase (ALT) | MyBioSource MBS2025844 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Alanine Aminotransferase (ALT)

catalog :

MBS2025844

quantity :

0.01 mg

price :

100 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[11#]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant ALT, Human.

image 2 :

DAB staining on IHC-P;Samples:Human Glioma Tissue.

image 3 :

DABstainingonIHC-P;Samples:HumanLiverCancerTissue.

product information

catalog number :

MBS2025844

products type :

Antibody

products full name :

Monoclonal Antibody to Alanine Aminotransferase (ALT)

products short name :

[Alanine Aminotransferase]

other names :

[alanine aminotransferase 1; Alanine aminotransferase 1; alanine aminotransferase 1; glutamic--pyruvic transaminase; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1]

products gene name :

[ALT]

other gene names :

[GPT; GPT; AAT1; ALT1; GPT1; AAT1; GPT1; ALT1; GPT 1]

clonality :

Monoclonal

isotype :

IgG2b Kappa

clone :

[11#]

host :

Mouse

purity :

Protein A + Protein G affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

500 Ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2ug/mL;1:250-1000. Immunohistochemistry: 5-20ug/mL;1:25-100. Immunocytochemistry: 5-20ug/mL;1:25-100. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant ALT (Ile255~Ser496) expressed in E.coli

ncbi gi num :

4885351

ncbi acc num :

NP_005300.1

ncbi gb acc num :

NM_005309.2

uniprot acc num :

P24298

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567)

ncbi summary :

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

uniprot summary :

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: cytosol. Molecular Function: L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding. Biological Process: amino acid biosynthetic process; L-alanine catabolic process

size1 :

0.01 mg

price1 :

100 USD

size2 :

0.02 mg

price2 :

110

size3 :

0.05 mg

price3 :

140

size4 :

0.1 mg

price4 :

170

size5 :

0.2 mg

price5 :

220

size6 :

1 mg

price6 :

495

more info or order :

MyBioSource product webpage