Monoclonal Antibody to Noggin (NOG) | MyBioSource MBS2025799 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Noggin (NOG)

catalog :

MBS2025799

quantity :

0.01 mg

price :

105 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot Rat Placenta Tissue Primary Ab: 2ug/mL Rabbit Anti-Human NOG Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab

image 2 :

Western Blot: Sample: Recombinant protein.

image 3 :

DAB staining on IHC-P;Samples:Human Liver Cancer Tissue.

product information

catalog number :

MBS2025799

products type :

Antibody

products full name :

Monoclonal Antibody to Noggin (NOG)

products short name :

[Noggin]

other names :

[noggin; Noggin; noggin; noggin]

products gene name :

[NOG]

other gene names :

[NOG; NOG; SYM1; SYNS1; SYNS1A]

clonality :

Monoclonal

isotype :

IgG2b Kappa

host :

Mouse

sequence length :

232

purity :

Protein A + Protein G affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting : 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

DAB staining on IHC-P Samples:Human Breast Cancer Tissue

image5 heading :

Immunohistochemistry (IHC)

image5 description :

DAB staining on IHC-P;Samples:Human Glioma Tissue.

other info1 :

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Clone number: A11. Traits: Liquid. Immunogen: Recombinant NOG (Gln28~Cys232 (Accession # Q13253)) expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2063455

ncbi gi num :

4885523

ncbi acc num :

NP_005441.1

ncbi gb acc num :

NM_005450.4

uniprot acc num :

Q13253

ncbi pathways :

BMP Receptor Signaling Pathway (137948); BMP Signalling And Regulation Pathway (198910); Cardiac Progenitor Differentiation Pathway (712094); Signal Transduction Pathway (477114); Signaling By BMP Pathway (106336); TGF Beta Signaling Pathway (198810); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475)

ncbi summary :

The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse. [provided by RefSeq, Jul 2008]

uniprot summary :

NOG: Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Defects in NOG are a cause of symphalangism proximal syndrome (SYM1). SYM1 is characterized by the hereditary absence of the proximal interphalangeal (PIP) joints (Cushing symphalangism). Severity of PIP joint involvement diminishes towards the radial side. Distal interphalangeal joints are less frequently involved and metacarpophalangeal joints are rarely affected whereas carpal bone malformation and fusion are common. In the lower extremities, tarsal bone coalition is common. Conducive hearing loss is seen and is due to fusion of the stapes to the petrous part of the temporal bone. Defects in NOG are the cause of multiple synostoses syndrome type 1 (SYNS1); also known as synostoses, multiple, with brachydactyly/symphalangism-brachydactyly syndrome. SYNS1 is characterized by tubular-shaped (hemicylindrical) nose with lack of alar flare, otosclerotic deafness, and multiple progressive joint fusions commencing in the hand. The joint fusions are progressive, commencing in the fifth proximal interphalangeal joint in early childhood (or at birth in some individuals) and progressing in an ulnar-to-radial and proximal- to-distal direction. With increasing age, ankylosis of other joints, including the cervical vertebrae, hips, and humeroradial joints, develop. Defects in NOG are the cause of tarsal-carpal coalition syndrome (TCC). TCC is an autosomal dominant disorder characterized by fusion of the carpals, tarsals and phalanges, short first metacarpals causing brachydactyly, and humeroradial fusion. TCC is allelic to SYM1, and different mutations in NOG can result in either TCC or SYM1 in different families. Defects in NOG are a cause of stapes ankylosis with broad thumb and toes (SABTS); also known as Teunissen- Cremers syndrome. SABTS is a congenital autosomal dominant disorder that includes hyperopia, a hemicylindrical nose, broad thumbs, great toes, and other minor skeletal anomalies but lacked carpal and tarsal fusion and symphalangism. Defects in NOG are the cause of brachydactyly type B2 (BDB2). BDB2 is a subtype of brachydactyly characterized by hypoplasia/aplasia of distal phalanges in combination with distal symphalangism, fusion of carpal/tarsal bones, and partial cutaneous syndactyly. Belongs to the noggin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q22. Cellular Component: extracellular region; extracellular space. Molecular Function: cytokine binding; protein binding; protein homodimerization activity. Biological Process: atrial cardiac muscle morphogenesis; axial mesoderm development; BMP signaling pathway; cartilage development; cell differentiation in hindbrain; dorsal/ventral pattern formation; embryonic digit morphogenesis; embryonic skeletal system development; endoderm formation; epithelial to mesenchymal transition; in utero embryonic development; limb development; mesoderm formation; middle ear morphogenesis; motor axon guidance; negative regulation of astrocyte differentiation; negative regulation of BMP signaling pathway; negative regulation of cardiac muscle cell proliferation; negative regulation of cell migration; negative regulation of osteoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; nervous system development; neural plate morphogenesis; neural tube closure; notochord morphogenesis; osteoblast differentiation; pituitary gland development; positive regulation of epithelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; skeletal system development; somatic stem cell maintenance; spinal cord development; wound healing. Disease: Brachydactyly, Type B2; Multiple Synostoses Syndrome 1; Stapes Ankylosis With Broad Thumb And Toes; Symphalangism, Proximal, 1a; Tarsal-carpal Coalition Syndrome

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

110

size3 :

0.05 mg

price3 :

145

size4 :

0.1 mg

price4 :

185

size5 :

0.2 mg

price5 :

235

size6 :

1 mg

price6 :

540

more info or order :

MyBioSource product webpage