Monoclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP) | MyBioSource MBS2025794 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP)

catalog :

MBS2025794

quantity :

0.01 mg

price :

130 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[A6]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot; Sample: Human Hela cell lysate; Primary Ab: 6ug/ml Mouse Anti-Multi-species PIIINP Antibody

image 2 :

Western Blot; Sample: Human HepG2 cell lysate;. Primary Ab: 5ug/ml Mouse Anti-Multi-species PIIINP Antibody. Secondary Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (MBS2086035)

image 3 :

Figure. DAB staining on IHC-P; Samples: Human Stomach Tissue.

product information

catalog number :

MBS2025794

products type :

Antibody

products full name :

Monoclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP)

products short name :

[Procollagen III N-Terminal Propeptide]

other names :

[collagen alpha-1(III) chain preproprotein; Collagen alpha-1(III) chain; collagen alpha-1(III) chain; collagen type III alpha 1 chain]

products gene name :

[PIIINP]

other gene names :

[COL3A1; COL3A1; EDS4A]

clonality :

isotype :

IgG2b Kappa

clone :

[A6]

host :

Mouse

purity :

Protein A + Protein G affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

0.55 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-5 ug/mL;1:110-1100. Immunohistochemistry: 5-20 ug/mL;1:28-110. Immunocytochemistry: 5-20 ug/mL;1:28-110. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Figure. DAB staining on IHC-P;Samples: Human Kidney Tissue

other info1 :

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Traits: Liquid. Immunogen: Synthetic Peptide, PIIINP conjugated to OVA.Target peptide sequence: RDVWKPEPCQICVCD

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2044370

ncbi gi num :

4502951

ncbi acc num :

NP_000081.1

ncbi gb acc num :

NM_000090.3

uniprot acc num :

P02461

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endothelins Pathway (137958); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795)

ncbi summary :

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

uniprot summary :

CO3A1: Collagen type III occurs in most soft connective tissues along with type I collagen. Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3); also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA). AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. Belongs to the fibrillar collagen family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q32.2. Cellular Component: collagen type III trimer; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space. Molecular Function: extracellular matrix structural constituent; integrin binding; metal ion binding; platelet-derived growth factor binding; protease binding; protein binding; SMAD binding. Biological Process: aging; cell-matrix adhesion; cerebral cortex development; collagen catabolic process; collagen fibril organization; extracellular matrix organization; gut development; heart development; integrin-mediated signaling pathway; negative regulation of immune response; negative regulation of neuron migration; peptide cross-linking; platelet activation; positive regulation of Rho protein signal transduction; regulation of immune response; response to cytokine; response to mechanical stimulus; response to radiation; skeletal system development; skin development; transforming growth factor beta receptor signaling pathway; wound healing. Disease: Ehlers-danlos Syndrome, Type Iii; Ehlers-danlos Syndrome, Type Iv, Autosomal Dominant

size1 :

0.01 mg

price1 :

130 USD

size2 :

0.02 mg

price2 :

140

size3 :

0.05 mg

price3 :

190

size4 :

0.1 mg

price4 :

245

size5 :

0.2 mg

price5 :

335

size6 :

1 mg

price6 :

785

more info or order :

MyBioSource product webpage