Monoclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17) | MyBioSource MBS2025765 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)

catalog :

MBS2025765

quantity :

0.01 mg

price :

90 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[C10]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Human U937 Cells.

image 2 :

Western Blot: Sample: Recombinant ADAM17, Human.

image 3 :

DAB staining on IHC-P. Samples: Human Skin cancer Tissue. Primary Ab: 20 ug/ml Mouse Anti? Human. ADAM17 Antibody . Second Ab: 2 ug/mL HRP-Linked Caprine. Anti-Mouse IgG Polyclonal . Antibody (Catalog: MBS2086035)

product information

catalog number :

MBS2025765

products type :

Antibody

products full name :

Monoclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)

products short name :

[A Disintegrin And Metalloprotease 17]

other names :

[disintegrin and metalloproteinase domain-containing protein 17 preproprotein; Disintegrin and metalloproteinase domain-containing protein 17; disintegrin and metalloproteinase domain-containing protein 17; ADAM metallopeptidase domain 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD_antigen: CD156b]

products gene name :

[ADAM17]

other gene names :

[ADAM17; ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1; CSVP; TACE; ADAM 17]

clonality :

Monoclonal

isotype :

IgG1 Kappa

clone :

[C10]

host :

Mouse

purity :

Protein A + Protein G affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant ADAM17 (Lys226~Arg473) expressed in E.coli

ncbi gi num :

73747889

ncbi acc num :

NP_003174.3

ncbi gb acc num :

NM_003183.5

uniprot acc num :

P78536

ncbi pathways :

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); BDNF Signaling Pathway (712093); Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway (771591); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593); Constitutive Signaling By NOTCH1 T(7;9)(NOTCH1:M1580_K2555) Translocation Mutant Pathway (771589); Cytokine Signaling In Immune System Pathway (366171)

ncbi summary :

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. [provided by RefSeq, Feb 2016]

uniprot summary :

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala- -Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described. Protein type: EC 3.4.24.86; Membrane protein, integral; Motility/polarity/chemotaxis; Protease. Chromosomal Location of Human Ortholog: 2p25.1. Cellular Component: actin cytoskeleton; apical plasma membrane; cell surface; cytoplasm; cytosol; focal adhesion; integral component of plasma membrane; intercellular junction; membrane; membrane raft; plasma membrane. Molecular Function: integrin binding; interleukin-6 receptor binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; Notch binding; PDZ domain binding; protein binding; SH3 domain binding. Biological Process: B cell differentiation; cell adhesion; cell adhesion mediated by integrin; cell motility; defense response to Gram-positive bacterium; epidermal growth factor receptor signaling pathway; germinal center formation; membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; negative regulation of transforming growth factor beta receptor signaling pathway; neutrophil mediated immunity; Notch receptor processing; Notch signaling pathway; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cellular component movement; positive regulation of chemokine production; positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle; positive regulation of epidermal growth factor-activated receptor activity; positive regulation of leukocyte chemotaxis; positive regulation of protein phosphorylation; positive regulation of T cell chemotaxis; positive regulation of transforming growth factor beta receptor signaling pathway; proteolysis; regulation of mast cell apoptotic process; response to drug; response to high density lipoprotein stimulus; response to hypoxia; response to lipopolysaccharide; spleen development; T cell differentiation in thymus; tumor necrosis factor-mediated signaling pathway; wound healing, spreading of epidermal cells. Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1

size1 :

0.01 mg

price1 :

90 USD

size2 :

0.02 mg

price2 :

100

size3 :

0.05 mg

price3 :

130

size4 :

0.1 mg

price4 :

160

size5 :

0.2 mg

price5 :

200

size6 :

1 mg

price6 :

430

more info or order :

MyBioSource product webpage