Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9) | MyBioSource MBS2025637 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

catalog :

MBS2025637

quantity :

0.01 mg

price :

90 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[16#]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Human U937 cell lysate; Primary Ab: 3ug/ml Mouse Anti-Human MMP9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)

image 2 :

Western Blot: Sample: Recombinant MMP9, Human.

image 3 :

Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: MMP9 knockout HepG2 cell lysate; Predicted MW: 76kDa Observed MW: 90kDa Primary Ab: 3ug/ml Mouse Anti-Human MMP9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)

product information

catalog number :

MBS2025637

products type :

Antibody

products full name :

Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

products short name :

[Matrix Metalloproteinase 9]

other names :

[matrix metalloproteinase-9 preproprotein; Matrix metalloproteinase-9; matrix metalloproteinase-9; matrix metallopeptidase 9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB]

products gene name :

[MMP9]

other gene names :

[MMP9; MMP9; GELB; CLG4B; MMP-9; MANDP2; CLG4B; MMP-9; GELB]

clonality :

Monoclonal

isotype :

IgG2b Kappa

clone :

[16#]

host :

Mouse

purity :

Protein A + Protein G affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-5 ug/mL;1:200-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Knockout Validation

other info1 :

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant MMP9 (Gly213~Ala399) expressed in E.coli

products categories :

Knock-Out Validation Antibody

ncbi gi num :

74272287

ncbi acc num :

NP_004985.2

ncbi gb acc num :

NM_004994.2

uniprot acc num :

P14780

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Angiogenesis Pathway (198772); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); CXCR4-mediated Signaling Events Pathway (137910); Collagen Formation Pathway (645288); Degradation Of Collagen Pathway (730309); Degradation Of Collagen Pathway (762258)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

uniprot summary :

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family. Protein type: EC 3.4.24.35; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular exosome; extracellular region; extracellular space; ficolin-1-rich granule lumen; proteinaceous extracellular matrix; tertiary granule lumen. Molecular Function: collagen binding; endopeptidase activity; identical protein binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; serine-type endopeptidase activity; zinc ion binding. Biological Process: cellular response to cadmium ion; cellular response to reactive oxygen species; collagen catabolic process; embryo implantation; endodermal cell differentiation; ephrin receptor signaling pathway; extracellular matrix disassembly; leukocyte migration; macrophage differentiation; negative regulation of apoptosis; negative regulation of cation channel activity; negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; negative regulation of intrinsic apoptotic signaling pathway; neutrophil degranulation; ossification; positive regulation of cell migration; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; positive regulation of protein phosphorylation; positive regulation of receptor binding; positive regulation of release of cytochrome c from mitochondria; positive regulation of vascular smooth muscle cell proliferation; proteolysis; skeletal system development. Disease: Metaphyseal Anadysplasia 2

size1 :

0.01 mg

price1 :

90 USD

size2 :

0.02 mg

price2 :

100

size3 :

0.05 mg

price3 :

130

size4 :

0.1 mg

price4 :

160

size5 :

0.2 mg

price5 :

200

size6 :

1 mg

price6 :

430

more info or order :

MyBioSource product webpage