ELISA/assay

Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) ELISA Kit

MBS2025592

24-Strip-Wells

270 USD

ELISA Kit

Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) ELISA Kit

[Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)]

[ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein]

[mesencephalic astrocyte-derived neurotrophic factor; Mesencephalic astrocyte-derived neurotrophic factor; mesencephalic astrocyte-derived neurotrophic factor; mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET]

[MANF]

[Manf; Manf; Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik; Armet]

MANF_MOUSE

Mouse

179

This assay has high sensitivity and excellent specificity for detection of MANF. No significant cross-reactivity or interference between MANF and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.055ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MANF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MANF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Tumor immunity; Neuroscience

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MANF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MANF. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MANF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MANF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MANF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

110625813

NP_083379.2

NM_029103.3

20,374 Da

ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region; nucleus. Molecular Function: growth factor activity. Biological Process: vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure; response to unfolded protein

24-Strip-Wells

270 USD

48-Strip-Wells

505

96-Strip-Wells

685

5x96-Strip-Wells

2770

10x96-Strip-Wells

5025