ELISA/assay

Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit

MBS2025504

48-Strip-Wells

625 USD

ELISA Kit

Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit

Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)

wingless-type MMTV integration site family, member 3A; Protein Wnt-3a; protein Wnt-3a; wingless-type MMTV integration site family, member 3A

WNT3A

WNT3A; WNT3A

WNT3A_HUMAN

Human

352

This assay has high sensitivity for detection of WNT3A. 100% cross-reactivity of WNT3A was observed among human, mouse and rat.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Due to the 95% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: 0.067ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT3A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT3A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT3A in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Due to the 95% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119590235

EAW69829.1

42,905 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Canonical Wnt Signaling Pathway (138032); Cardiac Progenitor Differentiation Pathway (712094); Class B/2 (Secretin Family Receptors) Pathway (1269570); DNA Damage Response (only ATM Dependent) Pathway (198827); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855); GPCR Ligand Binding Pathway (1269544); HTLV-I Infection Pathway (373901)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; early endosome membrane; Golgi lumen; endoplasmic reticulum lumen; extracellular region; plasma membrane. Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding. Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; cell proliferation in forebrain; positive regulation of receptor internalization; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; positive regulation of collateral sprouting in the absence of injury; palate development; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation

48-Strip-Wells

625 USD

96-Strip-Wells

855

5x96-Strip-Wells

3355

10x96-Strip-Wells

6035