ELISA/assay

Uridine Triphosphate (UTP) ELISA Kit

MBS2025220

24-Strip-Wells

295 USD

ELISA Kit

Uridine Triphosphate (UTP) ELISA Kit

[Uridine Triphosphate (UTP)]

[UTP]

General

This assay has high sensitivity for detection of ubiquitin. 100% cross-reactivity of ubiquitin was observed among human, rat, mouse, porcine, rabbit, equine, bovine, canine.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 4.94-400ng/mL. Sensitivity: 1.92ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ubiquitin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ubiquitin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ubiquitin in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 100% homology of the sequence among different species, the kit can be used to detect human, rat, mouse, porcine, rabbit, equine, bovine, canine samples. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ubiquitin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ubiquitin and unlabeled ubiquitin (Standards or samples) with the pre-coated antibody specific to ubiquitin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ubiquitin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ubiquitin in the sample.

24-Strip-Wells

295 USD

48-Strip-Wells

560

96-Strip-Wells

765

5x96-Strip-Wells

3105

10x96-Strip-Wells

5600