catalog number :
MBS2025217
products full name :
Polyclonal Antibody to Erythropoietin (EPO)
products short name :
[Erythropoietin (EPO)]
other names :
[erythropoietin; Erythropoietin; erythropoietin; erythropoietin]
products gene name :
[EPO]
other gene names :
[Epo; Epo]
specificity :
The antibody is a rabbit polyclonal antibody raised against EPO. It has been selected for its ability to recognize EPO in immunohistochemical staining and western blotting.
purity :
Antigen-specific Affinity Chromatography.
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
app notes :
Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
other info1 :
Source: Polyclonal antibody preparation. Traits: Liquid
other info2 :
Immunogen: Recombinant EPO (Pro28~Arg192) expressed in E.coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2035397
ncbi acc num :
NP_058697.1
ncbi gb acc num :
NM_017001.1
ncbi mol weight :
21,286 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198514); HIF-1 Signaling Pathway (695233); Hematopoietic Cell Lineage Pathway (83470); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83469); Jak-STAT Signaling Pathway (488); PI3K-Akt Signaling Pathway (692247); PI3K-Akt Signaling Pathway (692979)
ncbi summary :
acts as a regulator of erythropoiesis; may facilitate oxygen delivery in response to hypoxia [RGD, Feb 2006]
uniprot summary :
Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.