ELISA/assay

Insulin Like Growth Factor Binding Protein 7 (IGFBP7) ELISA Kit

MBS2025192

48-Strip-Wells

575 USD

ELISA Kit

Insulin Like Growth Factor Binding Protein 7 (IGFBP7) ELISA Kit

Insulin Like Growth Factor Binding Protein 7 (IGFBP7)

FSTL2; IGFBP-7v; MAC25; PSF; TAF; IGFBP-rP1; MAC25 protein; Prostacyclin-stimulating factor; PGI2-stimulating factor; Tumor-derived adhesion factor

Insulin-like growth factor binding protein 7; Insulin-like growth factor-binding protein 7; insulin-like growth factor-binding protein 7; insulin-like growth factor binding protein 7; IGFBP-rP1; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; TAF

IGFBP7

IGFBP7; IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1; MAC25; PSF; IBP-7; IGF-binding protein 7; IGFBP-7; TAF

IBP7_HUMAN

Rat

282

This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 7 (IGFBP7). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 7 (IGFBP7) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 2.5-160ng/mL. Sensitivity: Typically less than 1.1ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 7 (IGFBP7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 7 (IGFBP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 7 (IGFBP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Insulin Like Growth Factor Binding Protein 7 (IGFBP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

42542649

AAH66339.1

28,860 Da

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Senescence And Autophagy Pathway (198780); Senescence-Associated Secretory Phenotype (SASP) Pathway (905996)

This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]

IGFBP7: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Defects in IGFBP7 are the cause of retinal arterial macroaneurysm with supravalvular pulmonic stenosis (RAMSVPS). RAMSVPS is an autosomal recessive condition characterized by the bilateral appearance of beading along the major retinal arterial trunks, with the subsequent formation of macroaneurysms. Affected individuals also have supravalvular pulmonic stenosis, often requiring surgical correction. Protein type: Cell cycle regulation; Secreted, signal peptide; Cell adhesion; Secreted. Chromosomal Location of Human Ortholog: 4q12. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: insulin-like growth factor binding; protein binding. Biological Process: negative regulation of cell proliferation; response to retinoic acid; response to cortisol stimulus; response to heat; regulation of steroid biosynthetic process; regulation of cell growth; cell adhesion; inner ear development; embryo implantation; cellular response to hormone stimulus. Disease: Retinal Arterial Macroaneurysm With Supravalvular Pulmonic Stenosis

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3065

10x96-Strip-Wells

5510