Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit | MyBioSource MBS2025158 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit

catalog :

MBS2025158

quantity :

24-Strip-Wells

price :

265 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2025158

products type :

ELISA Kit

products full name :

Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit

products short name :

[Actin Alpha 2, Smooth Muscle (ACTa2)]

products name syn :

[ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein]

other names :

[actin, alpha 2, smooth muscle, aorta, isoform CRA_a; Actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha 2, smooth muscle, aorta]

products gene name :

[ACTa2]

other gene names :

[ACTA2; ACTA2; AAT6; ACTSA; MYMY5]

uniprot entry name :

D2JYH4_HUMAN

reactivity :

Human

sequence length :

377

specificity :

This assay has high sensitivity and excellent specificity for detection of ACP5. No significant cross-reactivity or interference between ACP5 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Rat Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.31ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ACP5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ACP5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Developmental science; Bone metabolism

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACP5 in rat serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ACP5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ACP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ACP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ACP5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119570540

ncbi acc num :

EAW50155.1

ncbi mol weight :

42,009 Da

ncbi pathways :

Muscle Contraction Pathway (106261); Myometrial Relaxation And Contraction Pathways (198759); Smooth Muscle Contraction Pathway (161025); Striated Muscle Contraction Pathway (198903); Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

ncbi summary :

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

uniprot summary :

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family. Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q23.3. Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton. Molecular Function: protein kinase binding; ATP binding. Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction. Disease: Multisystemic Smooth Muscle Dysfunction Syndrome; Moyamoya Disease 5; Aortic Aneurysm, Familial Thoracic 6

size1 :

24-Strip-Wells

price1 :

265 USD

size2 :

48-Strip-Wells

price2 :

490

size3 :

96-Strip-Wells

price3 :

655

size4 :

5x96-Strip-Wells

price4 :

2660

size5 :

10x96-Strip-Wells

price5 :

4795

more info or order :

MyBioSource product webpage