ELISA/assay

Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA Kit

MBS2025147

24-Strip-Wells

285 USD

ELISA Kit

Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA Kit

[Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)]

[ATX; ATX-X; LysoPLD; NPP2; PD-IALPHA; PDNP2; Autotaxin; Extracellular lysophospholipase D]

[Ectonucleotide pyrophosphatase/phosphodiesterase 2; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ectonucleotide pyrophosphatase/phosphodiesterase 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD]

[ENPP2]

[ENPP2; ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA; ATX; PDNP2; E-NPP 2; LysoPLD]

ENPP2_HUMAN

Rat

863

This assay has high sensitivity and excellent specificity for detection of EN2. No significant cross-reactivity or interference between EN2 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.113ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EN2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EN2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Enzyme & Kinase

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EN2 in human tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EN2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

22137786

AAH34961.1

101,968 Da

Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365)

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Lipid Metabolism - ether lipid; Phosphodiesterase; Secreted; EC 3.1.4.39; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 8q24.1. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane. Molecular Function: nucleotide diphosphatase activity; phosphodiesterase I activity; nucleic acid binding; zinc ion binding; hydrolase activity; calcium ion binding; transcription factor binding; scavenger receptor activity; alkylglycerophosphoethanolamine phosphodiesterase activity; lysophospholipase activity; polysaccharide binding. Biological Process: G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; phospholipid catabolic process; immune response; chemotaxis; cell motility; regulation of cell migration; regulation of angiogenesis; phosphate metabolic process

24-Strip-Wells

285 USD

48-Strip-Wells

530

96-Strip-Wells

715

5x96-Strip-Wells

2910

10x96-Strip-Wells

5280