catalog number :
MBS2025077
products type :
ELISA Kit
products full name :
Actin Beta (ACTb) ELISA Kit
products short name :
[Actin Beta (ACTb)]
products name syn :
[ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed]
other names :
[actin beta; actin beta]
products gene name :
[ACTb]
other gene names :
[actb; B-actin; beta-actin]
specificity :
This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Assay Type: Double-antibody Sandwich. Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 1.56-100ng/mL. Sensitivity: < 0.55ng/mL
other info2 :
Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Beta (ACTb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Beta (ACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
products categories :
Signal transduction; Developmental science; Bone metabolism
products description :
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Beta (ACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Beta (ACTb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Actin Beta (ACTb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_001117707.1
ncbi gb acc num :
NM_001124235.1
size5 :
10x96-Strip-Wells