ELISA/assay

Procollagen I N-Terminal Propeptide (PINP) ELISA Kit

MBS2024987

24-Strip-Wells

270 USD

ELISA Kit

Procollagen I N-Terminal Propeptide (PINP) ELISA Kit

[Procollagen I N-Terminal Propeptide (PINP)]

[P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide]

[PINP]

Rat

This assay has high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide (PINP). No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Assay Type: Competitive Inhibition. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 246.9-20,000pg/mL. Sensitivity: < 91.5pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Metabolic pathway; Hepatology; Bone metabolism

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen I N-Terminal Propeptide (PINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen I N-Terminal Propeptide (PINP) and unlabeled Procollagen I N-Terminal Propeptide (PINP) (Standards or samples) with the pre-coated antibody specific to Procollagen I N-Terminal Propeptide (PINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen I N-Terminal Propeptide (PINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen I N-Terminal Propeptide (PINP) in the sample.

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2705

10x96-Strip-Wells

4875