ELISA/assay

S-Adenosyl Methionine (SAM) ELISA Kit

MBS2024941

24-Strip-Wells

295 USD

ELISA Kit

S-Adenosyl Methionine (SAM) ELISA Kit

[S-Adenosyl Methionine (SAM)]

[SAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)-L-Methionine Chloride]

[SAM]

General

This assay has high sensitivity and excellent specificity for detection of S1P. No significant cross-reactivity or interference between S1P and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 12.35-1,000ng/mL. Sensitivity: 4.62ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S1P were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S1P were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of S1P in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to S1P has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled S1P and unlabeled S1P (Standards or samples) with the pre-coated antibody specific to S1P. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of S1P in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of S1P in the sample.

24-Strip-Wells

295 USD

48-Strip-Wells

560

96-Strip-Wells

765

5x96-Strip-Wells

3105

10x96-Strip-Wells

5600