Interleukin 18 (IL18) ELISA Kit | MyBioSource MBS2024682 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Interleukin 18 (IL18) ELISA Kit

catalog :

MBS2024682

quantity :

24-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2024682

products type :

ELISA Kit

products full name :

Interleukin 18 (IL18) ELISA Kit

products short name :

[Interleukin 18 (IL18)]

products name syn :

[IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma]

other names :

[interleukin-18 isoform 1 proprotein; Interleukin-18; interleukin-18; interleukin 18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma]

products gene name :

[IL18]

other gene names :

[IL18; IL18; IGIF; IL-18; IL-1g; IL1F4; IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma]

uniprot entry name :

IL18_HUMAN

reactivity :

Mouse

sequence length :

193

specificity :

This assay has high sensitivity and excellent specificity for detection of Interleukin 18 (IL18). No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 15.6-1000pg/mL. Sensitivity: < 5.9pg/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Cytokine; Infection immunity

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 (IL18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 (IL18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 (IL18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Interleukin 18 (IL18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4504653

ncbi acc num :

NP_001553.1

ncbi gb acc num :

NM_001562.3

uniprot acc num :

Q14116

ncbi mol weight :

21,896 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cellular Roles Of Anthrax Toxin Pathway (138076); Corticotropin-releasing Hormone Pathway (920957); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Hypertrophy Model Pathway (198828)

ncbi summary :

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

uniprot summary :

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 11q22.2-q22.3. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein binding; cytokine activity. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; regulation of cell adhesion; positive regulation of interleukin-17 production; T-helper 1 type immune response; positive regulation of natural killer cell proliferation; interleukin-2 biosynthetic process; T-helper 2 type immune response; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response

size1 :

24-Strip-Wells

price1 :

260 USD

size2 :

48-Strip-Wells

price2 :

465

size3 :

96-Strip-Wells

price3 :

625

size4 :

5x96-Strip-Wells

price4 :

2530

size5 :

10x96-Strip-Wells

price5 :

4565

more info or order :

MyBioSource product webpage