ELISA/assay

Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit

MBS2024660

48-Strip-Wells

625 USD

ELISA Kit

Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit

Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6)

TSG6; TNF-Stimulated Gene 6; Hyaluronate-binding protein; Tumor necrosis factor alpha-induced protein 6

tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor-inducible gene 6 protein; tumor necrosis factor-inducible gene 6 protein; tumor necrosis factor, alpha-induced protein 6; Hyaluronate-binding protein; TNF-stimulated gene 6 protein; TSG-6; Tumor necrosis factor alpha-induced protein 6; TNF alpha-induced protein 6

TNFaIP6

TNFAIP6; TNFAIP6; TSG6; TSG-6; TSG6; TSG-6; TNF alpha-induced protein 6

TSG6_HUMAN

Mouse

277

This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6). No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: Typically less than 30pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

26051243

NP_009046.2

NM_007115.3

P98066

31,203 Da

The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.[provided by RefSeq, Dec 2010]

TNFAIP6: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis. Chromosomal Location of Human Ortholog: 2q23.3. Cellular Component: extracellular space. Molecular Function: hyaluronic acid binding. Biological Process: ovulation; cell-cell signaling; signal transduction; inflammatory response; cell adhesion; positive regulation of cell migration

48-Strip-Wells

625 USD

96-Strip-Wells

855

5x96-Strip-Wells

3355

10x96-Strip-Wells

6035