ELISA/assay

CD5 Antigen Like Protein (CD5L) ELISA Kit

MBS2024653

24-Strip-Wells

285 USD

ELISA Kit

CD5 Antigen Like Protein (CD5L) ELISA Kit

[CD5 Antigen Like Protein (CD5L)]

[API6; CT-2; SP-ALPHA; Spalpha; Apoptosis Inhibitor 6; Scavenger Receptor Cysteine Rich Family; IgM-associated peptide]

[CD5 antigen-like; CD5 antigen-like; CD5 antigen-like; CD5 molecule-like; CT-2; IgM-associated peptide; SP-alpha]

[CD5L]

[CD5L; CD5L; AIM; API6; PRO229; Spalpha; SP-ALPHA; API6]

CD5L_HUMAN

Human

347

This assay has high sensitivity and excellent specificity for detection of CD45. No significant cross-reactivity or interference between CD45 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.058ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD45 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD45 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

CD & Adhesion molecule

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD45 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CD45. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CD45. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD45, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CD45 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

5174411

NP_005885.1

NM_005894.2

O43866

38,088 Da

CD5L: May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis. Protein type: Receptor, misc.; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q23.1. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: scavenger receptor activity. Biological Process: receptor-mediated endocytosis; apoptosis; cellular defense response

24-Strip-Wells

285 USD

48-Strip-Wells

535

96-Strip-Wells

725

5x96-Strip-Wells

2945

10x96-Strip-Wells

5345