Thymosin Beta 4 (Tb4) ELISA Kit | MyBioSource MBS2024602 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Thymosin Beta 4 (Tb4) ELISA Kit

catalog :

MBS2024602

quantity :

48-Strip-Wells

price :

540 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2024602

products type :

ELISA Kit

products full name :

Thymosin Beta 4 (Tb4) ELISA Kit

products short name :

Thymosin Beta 4 (Tb4)

products name syn :

TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide

other names :

thymosin beta-4; Thymosin beta-4; thymosin beta-4; thymosin beta 4, X-linked; Fx

products gene name :

Tb4

other gene names :

TMSB4X; TMSB4X; FX; TB4X; PTMB4; TMSB4; TB4X; THYB4; TMSB4; T beta-4

uniprot entry name :

TYB4_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Tb4. No significant cross-reactivity or interference between Tb4 and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 2.47-200ng/mL. The standard curve concentrations used for the ELISA's were 200ng/mL, 66.67ng/mL, 22.22ng/mL, 7.41ng/mL, 2.47ng/mL. Sensitivity: 0.91ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tb4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tb4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Tb4 in human serum, plasma and other biological fluids. Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tb4 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tb4 and unlabeled Tb4 (Standards or samples) with the pre-coated antibody specific to Tb4. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tb4 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tb4 in the sample.

ncbi gi num :

11056061

ncbi acc num :

NP_066932.1

ncbi gb acc num :

NM_021109.3

uniprot acc num :

P62328

ncbi mol weight :

5,053 Da

ncbi pathways :

Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

ncbi summary :

This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]

uniprot summary :

TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: Xq21.3-q22. Cellular Component: cytoskeleton; extracellular region. Molecular Function: actin monomer binding; protein binding. Biological Process: platelet activation; platelet degranulation; sequestering of actin monomers; actin filament organization; blood coagulation

size1 :

48-Strip-Wells

price1 :

540 USD

size2 :

96-Strip-Wells

price2 :

730

size3 :

5x96-Strip-Wells

price3 :

2830

size4 :

10x96-Strip-Wells

price4 :

5080

more info or order :

MyBioSource product webpage