Sphingosine Kinase 1 (SPHK1) ELISA Kit | MyBioSource MBS2024588 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sphingosine Kinase 1 (SPHK1) ELISA Kit

catalog :

MBS2024588

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2024588

products type :

ELISA Kit

products full name :

Sphingosine Kinase 1 (SPHK1) ELISA Kit

products short name :

Sphingosine Kinase 1 (SPHK1)

other names :

sphingosine kinase 1; Sphingosine kinase 1; sphingosine kinase 1

products gene name :

SPHK1

other gene names :

SPHK1; SPHK1; F18E5.160; F18E5_160; sphingosine kinase 1; SPHK1

uniprot entry name :

SPHK1_ARATH

reactivity :

Human

sequence length :

320

specificity :

This assay has high sensitivity and excellent specificity for detection of Sphingosine Kinase 1 (SPHK1). No significant cross-reactivity or interference between Sphingosine Kinase 1 (SPHK1) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.122ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine Kinase 1 (SPHK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine Kinase 1 (SPHK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine Kinase 1 (SPHK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine Kinase 1 (SPHK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine Kinase 1 (SPHK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Sphingosine Kinase 1 (SPHK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

334186773

ncbi acc num :

NP_193885.6

ncbi gb acc num :

NM_118274.7

uniprot acc num :

Q8L7L1

ncbi mol weight :

35,548 Da

ncbi pathways :

Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway (1118767); Chaperonin-mediated Protein Folding Pathway (1118762); Metabolic Pathways (132014); Metabolism Pathway (1118260); Metabolism Of Lipids And Lipoproteins Pathway (1118293); Metabolism Of Proteins Pathway (1118760); Protein Folding Pathway (1118761); Sphingolipid De Novo Biosynthesis Pathway (1118372); Sphingolipid Metabolism Pathway (4048); Sphingolipid Metabolism Pathway (1118371)

ncbi summary :

Encodes a sphingosine kinase, also has enzyme activity towards other plant long-chain sphingoid bases. Involved in guard cell ABA signalling and seed germination.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage