ELISA/assay

Eosinophil Peroxidase (EPX) ELISA Kit

MBS2024555

48-Strip-Wells

565 USD

ELISA Kit

Eosinophil Peroxidase (EPX) ELISA Kit

Eosinophil Peroxidase (EPX)

EPO; EPP; EPX-PEN; EPER

eosinophil peroxidase; Eosinophil peroxidase; eosinophil peroxidase; eosinophil peroxidase

EPX

EPX; EPX; EPO; EPP; EPX-PEN; EPER; EPO; EPP; EPO

PERE_HUMAN

Human

715

This assay has high sensitivity and excellent specificity for detection of Eosinophil Peroxidase (EPX). No significant cross-reactivity or interference between Eosinophil Peroxidase (EPX) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.25-80ng/mL. Sensitivity: Typically less than 0.53ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Peroxidase (EPX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Peroxidase (EPX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eosinophil Peroxidase (EPX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eosinophil Peroxidase (EPX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eosinophil Peroxidase (EPX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Eosinophil Peroxidase (EPX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

182146

AAA58458.1

P11678

81,040 Da

Asthma Pathway (83120); Asthma Pathway (532)

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded precursor protein is processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a cluster of three peroxidase genes at chromosome 17q23. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Sep 2009]

EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily. Protein type: Oxidoreductase; EC 1.11.1.7. Chromosomal Location of Human Ortholog: 17q23.1. Molecular Function: peroxidase activity; metal ion binding; heme binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; hydrogen peroxide catabolic process; negative regulation of interleukin-5 production; response to oxidative stress; defense response to nematode. Disease: Eosinophil Peroxidase Deficiency

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340