ELISA/assay

Oxytocin Receptor (OXTR) ELISA Kit

MBS2024520

48-Strip-Wells

605 USD

ELISA Kit

Oxytocin Receptor (OXTR) ELISA Kit

Oxytocin Receptor (OXTR)

OT-R

oxytocin receptor; Oxytocin receptor; oxytocin receptor; oxytocin receptor

OXTR

OXTR; OXTR; OT-R; OT-R

OXYR_HUMAN

Rat

389

This assay has high sensitivity and excellent specificity for detection of Oxytocin Receptor (OXTR). No significant cross-reactivity or interference between Oxytocin Receptor (OXTR) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.065ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxytocin Receptor (OXTR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxytocin Receptor (OXTR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxytocin Receptor (OXTR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxytocin Receptor (OXTR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxytocin Receptor (OXTR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Oxytocin Receptor (OXTR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

32307152

NP_000907.2

NM_000916.3

P30559

42,772 Da

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295)

The protein encoded by this gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition. [provided by RefSeq, Jul 2008]

OXTR: Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR. Chromosomal Location of Human Ortholog: 3p25. Cellular Component: microvillus; cell-cell adherens junction; integral to plasma membrane; apical plasma membrane; plasma membrane. Molecular Function: oxytocin receptor activity; peptide hormone binding; peptide binding; vasopressin receptor activity. Biological Process: lactation; response to peptide hormone stimulus; response to anoxia; heart development; female pregnancy; positive regulation of synaptic transmission, glutamatergic; response to estradiol stimulus; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; muscle contraction; response to drug; maternal behavior; eating behavior; response to amphetamine; positive regulation of synaptic transmission, GABAergic; social behavior; response to cocaine; sleep; memory; cellular response to hormone stimulus; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; telencephalon development; gut development; suckling behavior; regulation of systemic arterial blood pressure by vasopressin; response to cytokine stimulus; maternal process involved in parturition; positive regulation of vasoconstriction; sperm ejaculation; response to progesterone stimulus; positive regulation of blood pressure

48-Strip-Wells

605 USD

96-Strip-Wells

820

5x96-Strip-Wells

3220

10x96-Strip-Wells

5795