ELISA/assay

Immunoglobulin G (IgG) ELISA Kit

MBS2024515

24-Strip-Wells

205 USD

ELISA Kit

Immunoglobulin G (IgG) ELISA Kit

[Immunoglobulin G (IgG)]

[immunoglobulin G]

[IgG]

Mouse

464

This assay has high sensitivity and excellent specificity for detection of IgG. No significant cross-reactivity or interference between IgG and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Canine Serum, Plasma Or Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 1.56-100ug/mL. Sensitivity: 0.47ug/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IgG were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IgG were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Infection immunity; Immune molecule; Hematology

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IgG in canine serum, plasma or other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to IgG has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IgG and unlabeled IgG (Standards or samples) with the pre-coated antibody specific to IgG. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IgG in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IgG in the sample.

21538829

AAM61760.1

24-Strip-Wells

205 USD

48-Strip-Wells

315

96-Strip-Wells

410

5x96-Strip-Wells

1620

10x96-Strip-Wells

2910