ELISA/assay

Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit

MBS2024475

96 Strip Wells

780 USD

ELISA Kit

Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit

Ryanodine Receptor 1, Skeletal (RYR1)

CCO; MHS; MHS1; RYDR; RYR; SKRR; Central Core Disease Of Muscle; Skeletal muscle calcium release channel

ryanodine receptor 1, partial; Ryanodine receptor 1; ryanodine receptor 1; ryanodine receptor 1 (skeletal); Ryanodine receptor 1

RYR1

RYR1; RYR1; CCO; MHS; RYR; MHS1; RYDR; SKRR; RYR-1; PPP1R137

G9I593_HUMAN

Human

49

This assay has high sensitivity and excellent specificity for detection of Ryanodine Receptor 1, Skeletal (RYR1). No significant cross-reactivity or interference between Ryanodine Receptor 1, Skeletal (RYR1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Tissue homogenates and other biological fluids. Detection Range: 0.781-50ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 0.27ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ryanodine Receptor 1, Skeletal (RYR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ryanodine Receptor 1, Skeletal (RYR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ryanodine Receptor 1, Skeletal (RYR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ryanodine Receptor 1, Skeletal (RYR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ryanodine Receptor 1, Skeletal (RYR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Ryanodine Receptor 1, Skeletal (RYR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

365768791

AEW90767.1

5,641 Da

Calcium Regulation In The Cardiac Cell Pathway 198906!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Circadian Entrainment Pathway 698773!!Circadian Entrainment Pathway 699872!!Ion Channel Transport Pathway 1269950!!Long-term Depression Pathway 83086!!Long-term Depression Pathway 497!!Myometrial Relaxation And Contraction Pathways 198759!!Oxytocin Signaling Pathway 952859

This gene encodes a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

96 Strip Wells

780 USD