ELISA/assay

Microtubule Associated Protein Tau (MAPt) ELISA Kit

MBS2024442

48-Strip-Wells

575 USD

ELISA Kit

Microtubule Associated Protein Tau (MAPt) ELISA Kit

Microtubule Associated Protein Tau (MAPt)

DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; Neurofibrillary tangle protein; Tau Protein; Paired helical filament-tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1

microtubule-associated protein tau; Microtubule-associated protein tau; microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau

MAPt

MAPT; MAPT; tau; TAU; PHF-tau

TAU_BOVIN

Rat

416

This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein Tau (MAPt). No significant cross-reactivity or interference between Microtubule Associated Protein Tau (MAPt) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 7.81-500pg/mL. Sensitivity: Typically less than 3.1pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein Tau (MAPt) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein Tau (MAPt) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein Tau (MAPt). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein Tau (MAPt). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein Tau (MAPt), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Microtubule Associated Protein Tau (MAPt) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

27806547

NP_776531.1

NM_174106.2

P29172

43,806 Da

Alzheimer's Disease Pathway (84336); Alzheimer's Disease Pathway (509); Apoptosis Pathway (1119590); Apoptotic Cleavage Of Cellular Proteins Pathway (1119612); Apoptotic Execution Phase Pathway (1119611); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1119613); IL-6 Signaling Pathway (920925); MAPK Signaling Pathway (198550); MAPK Signaling Pathway (84287); MAPK Signaling Pathway (456)

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer s disease, Pick s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization. Protein type: Cytoskeletal. Cellular Component: microtubule; growth cone; axon; tubulin complex; plasma membrane; axoneme; cytosol. Molecular Function: protein binding; microtubule binding; apolipoprotein binding; SH3 domain binding; protein kinase binding. Biological Process: negative regulation of intracellular transport; axon extension; positive regulation of microtubule polymerization; positive regulation of axon extension; axon cargo transport; neuron migration; regulation of autophagy; microtubule cytoskeleton organization and biogenesis; adult walking behavior; mitochondrion transport along microtubule

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3065

10x96-Strip-Wells

5510