Rat Relaxin 3 (RLN3) ELISA Kit | MyBioSource MBS2024441 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Relaxin 3 (RLN3) ELISA Kit

catalog :

MBS2024441

quantity :

48-Strip-Wells

price :

595 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2024441

products type :

ELISA Kit

products full name :

Rat Relaxin 3 (RLN3) ELISA Kit

products short name :

Relaxin 3 (RLN3)

other names :

relaxin-3 preproprotein; Relaxin-3; relaxin-3; prorelaxin H3; insulin-like 7; insulin-like peptide 7; insulin-like peptide INSL7; relaxin 3; Insulin-like peptide INSL7; Insulin-like peptide 7; Prorelaxin H3

products gene name :

RLN3

other gene names :

RLN3; RLN3; H3; RXN3; ZINS4; insl7; INSL7; RXN3; ZINS4; Insulin-like peptide 7

uniprot entry name :

REL3_HUMAN

reactivity :

Rat

sequence length :

142

specificity :

This assay has high sensitivity and excellent specificity for detection of RLN3. No significant cross-reactivity or interference between RLN3 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Rat serum, plasma and other biological fluids. Detection Range: 31.2-2,000pg/mL. Sensitivity: The minimum detectable dose of rat RLN3 is typically less than 11.5pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RLN3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RLN3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RLN3 in rat serum, plasma and other biological fluids.

products description :

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RLN3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RLN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RLN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RLN3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

18254464

ncbi acc num :

NP_543140.1

ncbi gb acc num :

NM_080864.2

uniprot acc num :

Q8WXF3

ncbi mol weight :

15,451 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Relaxin Receptors Pathway (161022); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

This gene encodes a member of the relaxin family of insulin-like hormones that is expressed predominantly in the brain and plays a role in physiological processes such as stress, memory and appetite regulation. The encoded protein is a precursor that is proteolytically processed to generate a heterodimeric mature form consisting A and B chains interlinked by disulfide bonds. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

uniprot summary :

RLN3: May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142). Belongs to the insulin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular region. Molecular Function: protein binding; G-protein-coupled receptor binding; hormone activity

size1 :

48-Strip-Wells

price1 :

595 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

3220

size4 :

10x96-Strip-Wells

price4 :

5795

more info or order :

MyBioSource product webpage