ELISA/assay

Platelet Activating Factor (PAF) ELISA Kit

MBS2024376

24-Strip-Wells

290 USD

ELISA Kit

Platelet Activating Factor (PAF) ELISA Kit

[Platelet Activating Factor (PAF)]

[PAF-Acether; AGEPC; Acetyl-Glyceryl-Ether-Phosphorylcholine]

[platelet activating factor; platelet activating factor]

[PAF]

[SAG1104]

General

204

This assay has high sensitivity and excellent specificity for detection of oxytocin. No significant cross-reactivity or interference between oxytocin and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 1.37-1,000pg/mL. Sensitivity: 0.53pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level oxytocin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level oxytocin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Metabolic pathway; Infection immunity; Cardiovascular biology

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of oxytocin in serum, plasma other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to oxytocin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled oxytocin and unlabeled oxytocin (Standards or samples) with the pre-coated antibody specific to oxytocin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of oxytocin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of oxytocin in the sample.

22537262

NP_688113.1

NC_004116.1

24-Strip-Wells

290 USD

48-Strip-Wells

550

96-Strip-Wells

745

5x96-Strip-Wells

3035

10x96-Strip-Wells

5475