ELISA/assay

Membrane Cofactor Protein (MCP) ELISA Kit

MBS2024320

24-Strip-Wells

260 USD

ELISA Kit

Membrane Cofactor Protein (MCP) ELISA Kit

[Membrane Cofactor Protein (MCP)]

[CD46; MIC10; TLX; TRA2; Trophoblast leukocyte common antigen; CD46 Molecule,Complement Regulatory Protein; CD46 Molecule,Complement Regulatory protein]

[membrane cofactor protein; Membrane cofactor protein; membrane cofactor protein; CD46 molecule, complement regulatory protein; TLX; Trophoblast leukocyte common antigen; CD_antigen: CD46]

[MCP]

[CD46; CD46; MCP; TLX; AHUS2; MIC10; TRA2.10; MCP; MIC10]

MCP_HUMAN

Human

336

This assay has high sensitivity and excellent specificity for detection of Membrane Cofactor Protein (MCP). No significant cross-reactivity or interference between Membrane Cofactor Protein (MCP) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Urine, Saliva and Other Biological Fluids. Detection Range: 12.5-800pg/mL. Sensitivity: < 4.8pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Membrane Cofactor Protein (MCP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Membrane Cofactor Protein (MCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

CD & Adhesion molecule

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Cofactor Protein (MCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Membrane Cofactor Protein (MCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Cofactor Protein (MCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Membrane Cofactor Protein (MCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

262938

AAB24802.1

P15529

36,826 Da

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Innate Immune System Pathway (106387); Measles Pathway (213306); Measles Pathway (213277); Regulation Of Complement Cascade Pathway (576254)

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc.; Cell surface. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: Golgi apparatus; inner acrosomal membrane; cell surface; focal adhesion; integral to plasma membrane; basolateral plasma membrane; plasma membrane. Molecular Function: complement binding; enzyme inhibitor activity; protein binding; cadherin binding; endopeptidase activity; receptor activity. Biological Process: positive regulation of memory T cell differentiation; adaptive immune response; positive regulation of regulatory T cell differentiation; viral reproduction; T cell mediated immunity; proteolysis; positive regulation of interleukin-10 production; regulation of Notch signaling pathway; negative regulation of complement activation; single fertilization; negative regulation of catalytic activity; interleukin-10 production; regulation of complement activation; innate immune response; positive regulation of T cell proliferation; complement activation, classical pathway

24-Strip-Wells

260 USD

48-Strip-Wells

475

96-Strip-Wells

640

5x96-Strip-Wells

2590

10x96-Strip-Wells

4670