ELISA/assay

Serum Amyloid A (SAA) ELISA Kit

MBS2024318

24-Strip-Wells

285 USD

ELISA Kit

Serum Amyloid A (SAA) ELISA Kit

[Serum Amyloid A (SAA)]

[SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein]

[serum amyloid A protein; serum amyloid A protein]

[SAA]

[SAA1; SAA]

Bovine

129

This assay has high sensitivity and excellent specificity for detection of S100B. No significant cross-reactivity or interference between S100B and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12-200pg/mL. Sensitivity: 1.22pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Infection immunity; Kidney biomarker; Hepatology

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100B in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to S100B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to S100B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

93352563

NP_001035288.1

NM_001040198.1

24-Strip-Wells

285 USD

48-Strip-Wells

525

96-Strip-Wells

715

5x96-Strip-Wells

2895

10x96-Strip-Wells

5255