Casein Beta (CSN2) ELISA Kit | MyBioSource MBS2024311 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Casein Beta (CSN2) ELISA Kit

catalog :

MBS2024311

quantity :

48-Strip-Wells

price :

655 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2024311

products type :

ELISA Kit

products full name :

Casein Beta (CSN2) ELISA Kit

products short name :

Casein Beta (CSN2)

products name syn :

CASb

other names :

Casein beta; Beta-casein; beta-casein; casein beta

products gene name :

CSN2

other gene names :

CSN2; CSN2; CASB; CASB

uniprot entry name :

CASB_HUMAN

reactivity :

Bovine

sequence length :

226

specificity :

This assay has high sensitivity and excellent specificity for detection of Casein Beta (CSN2). No significant cross-reactivity or interference between Casein Beta (CSN2) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Milk. Assay Type: Sandwich. Detection Range: 78.13-5000ng/mL. Sensitivity: Typically less than 28ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Casein Beta (CSN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Casein Beta (CSN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Beta (CSN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Beta (CSN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Beta (CSN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Casein Beta (CSN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

64654755

ncbi acc num :

AAH96197.1

ncbi mol weight :

25,382 Da

ncbi pathways :

Glucocorticoid Receptor Regulatory Network Pathway (138014); Nuclear Signaling By ERBB4 Pathway (530744); Prolactin Signaling Pathway (814182); Prolactin Signaling Pathway (814248); Signal Transduction Pathway (477114); Signaling By ERBB4 Pathway (530741); Signaling Events Mediated By PTP1B Pathway (138017)

ncbi summary :

This gene is a member of the beta casein family. There are two types of casein protein, beta (encoded by this gene) and kappa, both of which are secreted in human milk. Beta casein is the principal protein in human milk and the primary source of essential amino acids for a suckling infant. Beta and kappa casein proteins acting together form spherical micelles which bind within them important dietary minerals, such as calcium and phosphorous. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

uniprot summary :

CSN2: Important role in determination of the surface properties of the casein micelles. Belongs to the beta-casein family. Protein type: Secreted, signal peptide; Secreted; Calcium-binding; Inhibitor. Chromosomal Location of Human Ortholog: 4q21.1. Cellular Component: extracellular space; extracellular region. Molecular Function: enzyme inhibitor activity; transporter activity; calcium ion binding. Biological Process: lactation; calcium ion transport; negative regulation of catalytic activity

size1 :

48-Strip-Wells

price1 :

655 USD

size2 :

96-Strip-Wells

price2 :

900

size3 :

5x96-Strip-Wells

price3 :

3550

size4 :

10x96-Strip-Wells

price4 :

6350

more info or order :

MyBioSource product webpage