ELISA/assay

Vanin 1 (VNN1) ELISA Kit

MBS2024307

48-Strip-Wells

565 USD

ELISA Kit

Vanin 1 (VNN1) ELISA Kit

Vanin 1 (VNN1)

Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1

vanin 1; Pantetheinase; pantetheinase; vanin 1; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1; Vanin-1

VNN1

VNN1; VNN1; HDLCQ8; Tiff66; Vanin-1

VNN1_HUMAN

Human

513

This assay has high sensitivity and excellent specificity for detection of Vanin 1 (VNN1). No significant cross-reactivity or interference between Vanin 1 (VNN1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, urine and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.056ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vanin 1 (VNN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vanin 1 (VNN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vanin 1 (VNN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vanin 1 (VNN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vanin 1 (VNN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Vanin 1 (VNN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119568405

EAW48020.1

O95497

57,012 Da

Pantothenate And CoA Biosynthesis Pathway (83015); Pantothenate And CoA Biosynthesis Pathway (401)

This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. [provided by RefSeq, Feb 2009]

VNN1: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Belongs to the CN hydrolase family. BTD/VNN subfamily. Protein type: Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Membrane protein, integral; Hydrolase; Cell adhesion; Motility/polarity/chemotaxis; EC 3.5.1.92. Chromosomal Location of Human Ortholog: 6q23-q24. Cellular Component: integral to membrane; plasma membrane. Molecular Function: pantetheine hydrolase activity. Biological Process: positive regulation of T cell differentiation in the thymus; cell-cell adhesion; chronic inflammatory response; pantothenate metabolic process; innate immune response; response to oxidative stress; cell motility; inflammatory response; acute inflammatory response

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340