ELISA/assay

Claudin 5 (CLDN5) ELISA Kit

MBS2024302

48-Strip-Wells

565 USD

ELISA Kit

Claudin 5 (CLDN5) ELISA Kit

Claudin 5 (CLDN5)

AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS

claudin-5; Claudin-5; claudin-5; claudin 5; Transmembrane protein deleted in VCFS; TMDVCF

CLDN5

CLDN5; CLDN5; AWAL; BEC1; TMVCF; CPETRL1; AWAL; TMVCF; TMDVCF

CLD5_HUMAN

Human

218

This assay has high sensitivity and excellent specificity for detection of Claudin 5 (CLDN5). No significant cross-reactivity or interference between Claudin 5 (CLDN5) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Typically less than 13.3pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Claudin 5 (CLDN5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Claudin 5 (CLDN5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 5 (CLDN5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 5 (CLDN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 5 (CLDN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Claudin 5 (CLDN5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

195947374

NP_001124333.1

NM_001130861.1

O00501

23,147 Da

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494)

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]

Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family. Protein type: Membrane protein, multi-pass; Cytoskeletal; Membrane protein, integral. Chromosomal Location of Human Ortholog: 22q11.21. Cellular Component: tight junction; plasma membrane; integral to membrane; intercellular junction. Molecular Function: identical protein binding; structural molecule activity. Biological Process: calcium-independent cell-cell adhesion

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340