ELISA/assay

Interferon Alpha 2 (IFNa2) ELISA Kit

MBS2024295

96 Strip Wells

710 USD

ELISA Kit

Interferon Alpha 2 (IFNa2) ELISA Kit

Interferon Alpha 2 (IFNa2)

IFNA2A; IFNA2B; IFNA2C; LeIF A; Alpha-2a Interferon; Interferon Alpha 2b; Interferon Alpha A

interferon alpha-2; Interferon alpha-2; interferon alpha-2; interferon, alpha 2; Interferon alpha-A; LeIF A

IFNa2

IFNA2; IFNA2; IFNA; INFA2; IFNA2B; IFN-alphaA; IFNA2A; IFNA2B; IFNA2C; IFN-alpha-2; LeIF A

IFNA2_HUMAN

Human

188

This assay has high sensitivity and excellent specificity for detection of Interferon Alpha 2 (IFNa2). No significant cross-reactivity or interference between Interferon Alpha 2 (IFNa2) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 15.63-1000pg/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 6.2pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 2 (IFNa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha 2 (IFNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha 2 (IFNa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Interferon Alpha 2 (IFNa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

11067751

NP_000596.2

NM_000605.3

P01563

21,550 Da

Autoimmune Thyroid Disease Pathway 83121!!Autoimmune Thyroid Disease Pathway 533!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytosolic DNA-sensing Pathway 125137!!Cytosolic DNA-sensing Pathway 124833!!Factors Involved In Megakaryocyte Development And Platelet Production Pathway 187196!!Hemostasis Pathway 106028!!Hepatitis B Pathway 694606

This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Use of the recombinant form of this protein has been shown to be effective in reducing the symptoms and duration of the common cold. [provided by RefSeq, Jun 2011]

96 Strip Wells

710 USD