ELISA/assay

ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) ELISA Kit

MBS2024213

24-Strip-Wells

270 USD

ELISA Kit

ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) ELISA Kit

[ATPase, Cu++ Transporting Beta Polypeptide (ATP7b)]

[PWD; WC1; WD; WND; Wilson Disease Protein; Copper pump 2; Wilson disease-associated protein]

[ATPase, Cu++ transporting, beta polypeptide, isoform CRA_d; Copper-transporting ATPase 2; copper-transporting ATPase 2; ATPase, Cu++ transporting, beta polypeptide; Copper pump 2; Wilson disease-associated protein]

[ATP7b]

[ATP7B; ATP7B; WD; PWD; WC1; WND; PWD; WC1; WND]

ATP7B_HUMAN

Human

1,447

This assay has high sensitivity and excellent specificity for detection of ATP7b. No significant cross-reactivity or interference between ATP7b and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between ATP7b and all the analogues, therefore, cross reaction may still exist.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Tissue homogenates, cell lysates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.054ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP7b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP7b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATP7b in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ATP7b. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ATP7b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP7b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP7b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119629300

EAX08895.1

P35670

155,125 Da

Ion Channel Transport Pathway (187193); Ion Transport By P-type ATPases Pathway (187194); Transmembrane Transport Of Small Molecules Pathway (106572)

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]

ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter, ion channel; Membrane protein, multi-pass; EC 3.6.3.54; Vesicle; Hydrolase; Transporter; Membrane protein, integral. Chromosomal Location of Human Ortholog: 13q14.3. Cellular Component: Golgi membrane; membrane; mitochondrion; basolateral plasma membrane; integral to plasma membrane; perinuclear region of cytoplasm; late endosome; cytoplasmic membrane-bound vesicle; trans-Golgi network. Molecular Function: protein binding; copper ion binding; copper-exporting ATPase activity; ATP binding. Biological Process: lactation; cellular copper ion homeostasis; response to copper ion; metabolic process; cellular zinc ion homeostasis; sequestering of calcium ion; copper ion import; copper ion transport; transmembrane transport; copper ion export; intracellular copper ion transport. Disease: Wilson Disease

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895