C-Type Natriuretic Peptide (CNP) ELISA Kit | MyBioSource MBS2024183 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

C-Type Natriuretic Peptide (CNP) ELISA Kit

catalog :

MBS2024183

quantity :

24-Strip-Wells

price :

305 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2024183

products type :

ELISA Kit

products full name :

C-Type Natriuretic Peptide (CNP) ELISA Kit

products short name :

[C-Type Natriuretic Peptide (CNP)]

products name syn :

[NPPC; Natriuretic Peptide Precursor C]

other names :

[C-type natriuretic peptide; C-type natriuretic peptide; C-type natriuretic peptide; natriuretic peptide C]

products gene name :

[CNP]

other gene names :

[NPPC; NPPC; CNP; CNP2; CNP2]

uniprot entry name :

ANFC_HUMAN

reactivity :

Human

sequence length :

126

specificity :

This assay has high sensitivity and excellent specificity for detection of CNP. No significant cross-reactivity or interference between CNP and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5-4,000pg/mL. Sensitivity: <23.5pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Cardiovascular biology; Hormone metabolism

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CNP in human serum, plasma and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CNP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

13249346

ncbi acc num :

NP_077720.1

ncbi gb acc num :

NM_024409.2

uniprot acc num :

P23582

ncbi mol weight :

13,246 Da

ncbi summary :

The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]

uniprot summary :

NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family. Protein type: Secreted; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q37.1. Cellular Component: extracellular space; secretory granule. Molecular Function: protein homodimerization activity; peptide hormone receptor binding; hormone activity; receptor binding. Biological Process: response to drug; cGMP biosynthetic process; ossification; regulation of smooth muscle cell proliferation; regulation of cAMP metabolic process; regulation of multicellular organism growth; negative regulation of meiotic cell cycle; negative regulation of DNA metabolic process; positive regulation of vasodilation; post-embryonic development; positive regulation of osteoblast differentiation; negative regulation of cell proliferation; response to ethanol; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to hypoxia; regulation of cGMP metabolic process; regulation of blood vessel size

size1 :

24-Strip-Wells

price1 :

305 USD

size2 :

48-Strip-Wells

price2 :

575

size3 :

96-Strip-Wells

price3 :

785

size4 :

5x96-Strip-Wells

price4 :

3190

size5 :

10x96-Strip-Wells

price5 :

5755

more info or order :

MyBioSource product webpage