Periostin (POSTN) ELISA Kit | MyBioSource MBS2024123 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Periostin (POSTN) ELISA Kit

catalog :

MBS2024123

quantity :

48-Strip-Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2024123

products type :

ELISA Kit

products full name :

Periostin (POSTN) ELISA Kit

products short name :

Periostin (POSTN)

products name syn :

OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2

other names :

periostin isoform 1; Periostin; periostin; periostin, osteoblast specific factor; Osteoblast-specific factor 2; OSF-2

products gene name :

POSTN

other gene names :

POSTN; POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin; OSF2; PN; OSF-2

uniprot entry name :

POSTN_HUMAN

reactivity :

Mouse

sequence length :

721

specificity :

This assay has high sensitivity and excellent specificity for detection of Periostin (POSTN). No significant cross-reactivity or interference between Periostin (POSTN) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 46.88-3000pg/mL. Sensitivity: Typically less than 21.57pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Periostin (POSTN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Periostin (POSTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Periostin (POSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Periostin (POSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Periostin (POSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Periostin (POSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

209862907

ncbi acc num :

NP_006466.2

ncbi gb acc num :

NM_006475.2

uniprot acc num :

Q15063

ncbi mol weight :

80,357 Da

ncbi summary :

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

uniprot summary :

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 13q13.3. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; trans-Golgi network. Molecular Function: heparin binding; protein binding; cell adhesion molecule binding. Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development

size1 :

48-Strip-Wells

price1 :

575 USD

size2 :

96-Strip-Wells

price2 :

785

size3 :

5x96-Strip-Wells

price3 :

3055

size4 :

10x96-Strip-Wells

price4 :

5495

more info or order :

MyBioSource product webpage