ELISA/assay

Human Signal Transducer And Activator Of Transcription 3 (STAT3) ELISA Kit

MBS2024094

24-Strip-Wells

270 USD

ELISA Kit

Human Signal Transducer And Activator Of Transcription 3 (STAT3) ELISA Kit

[Signal Transducer And Activator Of Transcription 3 (STAT3)]

[signal transducer and activator of transcription 3; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3]

[STAT3]

[STAT3; STAT3]

STAT3_PIG

Human

770

This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 3 (STAT3). No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 3 (STAT3) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Standard Curve/Testing Data

Assay Type: Double-antibody Sandwich. Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.062ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 3 (STAT3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 3 (STAT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Signal transduction; Apoptosis

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 3 (STAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 3 (STAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 3 (STAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Signal Transducer And Activator Of Transcription 3 (STAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

113205774

NP_001038045.1

NM_001044580.1

Q19S50

88,067 Da

Acute Myeloid Leukemia Pathway (84524); Acute Myeloid Leukemia Pathway (529); Adipocytokine Signaling Pathway (84504); Adipocytokine Signaling Pathway (505); Chemokine Signaling Pathway (98758); Chemokine Signaling Pathway (96864); Cytokine Signaling In Immune System Pathway (938918); Disease Pathway (939771); Downstream Signal Transduction Pathway (939482); Epstein-Barr Virus Infection Pathway (585586)

Function: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA . By similarity. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity . By similarity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G . By similarity. Subunit structure: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1. Interacts with IL23R in presence of IL23. Interacts (via SH2 domain) with NLK. Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import. Interacts with CAV2; the interaction is increased on insulin-induced tyrosine phosphorylation of CAV2 and leads to STAT3 activation. Interacts with ARL2BP; interaction is enhanced with ARL2. Interacts with ARL2BP; the interaction is enhanced by LIF and JAK1 expression. Interacts with NEK6. Binds to CDK9 when activated and nuclear. Interacts with BMX. Interacts with EGFR; mediates EGFR downstream signaling to control cell proliferation. Interacts with STMN3, antagonizing its microtubule-destabilizing activity . By similarity. Interacts with the 'Lys-129' acetylated form of BIRC5/survivin. Interacts with FER . By similarity. Interacts with NEK6 . By similarity. Interacts with BMX . By similarity. Interacts with ZIPK/DAPK3 . By similarity. Interacts (via SH2 domain) with EIF2AK2/PKR (via the kinase catalytic domain) . By similarity. Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3 . By similarity. Ref.1. Tissue specificity: Detected in lung, heart, oviduct, ovary, uterus and kidney (at protein level). Detected in ovary, oviduct, and at lower levels in uterus and lung. Ref.1. Post-translational modification: Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, CNTF, LIF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Tyrosine phosphorylated upon stimulation with EGF. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Phosphorylated on Ser-727 by RPS6KA5 . By similarity. Phosphoryation at Tyr-705 by FER or PTK6 leads to an increase of its transcriptional activity . By similarity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling . By similarity. Ref.1. Miscellaneous: Involved in the gp130-mediated signaling pathway. Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895