Rat Fibroblast Growth Factor 21 (FGF21) ELISA Kit | MyBioSource MBS2024083 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Fibroblast Growth Factor 21 (FGF21) ELISA Kit

catalog :

MBS2024083

quantity :

48-Strip-Wells

price :

595 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2024083

products type :

ELISA Kit

products full name :

Rat Fibroblast Growth Factor 21 (FGF21) ELISA Kit

products short name :

Fibroblast Growth Factor 21 (FGF21)

other names :

fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; FGF-21; fibroblast growth factor 21

products gene name :

FGF21

other gene names :

FGF21; FGF21; FGF-21

uniprot entry name :

FGF21_HUMAN

reactivity :

Rat

sequence length :

209

specificity :

This assay has high sensitivity and excellent specificity for detection of FGF21. No significant cross-reactivity or interference between FGF21 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Rat serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Detection Range: 7.8-500pg/mL. Sensitivity: The minimum detectable dose of FGF21 is typically less than 2.9pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF21 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF21 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FGF21 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

products description :

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGF21. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FGF21. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FGF21, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FGF21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

9506597

ncbi acc num :

NP_061986.1

ncbi gb acc num :

NM_019113.2

uniprot acc num :

Q9NSA1

ncbi mol weight :

22,300 Da

ncbi pathways :

MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Melanoma Pathway (83114); Melanoma Pathway (526); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054); Rap1 Signaling Pathway (868086)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: extracellular region. Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding. Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction

size1 :

48-Strip-Wells

price1 :

595 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

3220

size4 :

10x96-Strip-Wells

price4 :

5795

more info or order :

MyBioSource product webpage