ELISA/assay

Nucleophosmin (NPM) ELISA Kit

MBS2024060

48-Strip-Wells

565 USD

ELISA Kit

Nucleophosmin (NPM) ELISA Kit

Nucleophosmin (NPM)

B23; NPM1; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family,Member 1; Nucleolar protein NO38

nucleophosmin; Nucleophosmin; nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

NPM

NPM1; NPM1; B23; NPM; NPM; NPM

NPM_HUMAN

Human

259

This assay has high sensitivity and excellent specificity for detection of NPM. No significant cross-reactivity or interference between NPM and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.312-20ng/mL. The standard curve concentrations used for the ELISA's were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL. Sensitivity: 0.122ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPM were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPM were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NPM in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NPM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NPM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NPM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

189272

AAA36380.1

P06748

28,400 Da

Aurora B Signaling Pathway (138080); BARD1 Signaling Events Pathway (137959); Cell Cycle Pathway (530733); Chromosome Maintenance Pathway (161048); Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway (161050); Disease Pathway (530764); HIF-1-alpha Transcription Factor Network Pathway (138045); HIV Infection Pathway (105983); Host Interactions Of HIV Factors Pathway (106010); Interactions Of Rev With Host Cellular Proteins Pathway (106014)

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described. Protein type: Translation; Oncoprotein; Nucleolus; RNA-binding. Chromosomal Location of Human Ortholog: 5q35.1. Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; nucleus; ribonucleoprotein complex. Molecular Function: NF-kappaB binding; protein binding; protein homodimerization activity; protein kinase inhibitor activity; histone binding; Tat protein binding; RNA binding; protein heterodimerization activity; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal small subunit binding; ribosomal large subunit binding. Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; signal transduction; DNA repair; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; negative regulation of cell proliferation; nucleosome assembly; protein localization; regulation of endodeoxyribonuclease activity; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340