Interferon Beta (IFNb) ELISA Kit | MyBioSource MBS2024041 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Interferon Beta (IFNb) ELISA Kit

catalog :

MBS2024041

quantity :

48-Strip-Wells

price :

550 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2024041

products type :

ELISA Kit

products full name :

Interferon Beta (IFNb) ELISA Kit

products short name :

Interferon Beta (IFNb)

products name syn :

IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast

other names :

interferon beta; Interferon beta; interferon beta; interferon, beta 1, fibroblast; Fibroblast interferon

products gene name :

IFNb

other gene names :

IFNB1; IFNB1; IFB; IFF; IFNB; IFB; IFNB; IFN-beta

uniprot entry name :

IFNB_HUMAN

reactivity :

Chicken

sequence length :

187

specificity :

This assay has high sensitivity and excellent specificity for detection of IFNb. No significant cross-reactivity or interference between IFNb and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.6-1,000pg/mL. The standard curve concentrations used for the ELISA's were 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL. Sensitivity: 6.2pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFNb were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFNb were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFNb in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFNb. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IFNb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4504603

ncbi acc num :

NP_002167.1

ncbi gb acc num :

NM_002176.2

uniprot acc num :

P01574

ncbi mol weight :

22,294 Da

ncbi pathways :

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833)

uniprot summary :

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to virus; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; cell surface receptor linked signal transduction; negative regulation of viral genome replication; B cell differentiation; defense response to bacterium; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response

size1 :

48-Strip-Wells

price1 :

550 USD

size2 :

96-Strip-Wells

price2 :

750

size3 :

5x96-Strip-Wells

price3 :

2910

size4 :

10x96-Strip-Wells

price4 :

5220

more info or order :

MyBioSource product webpage