Fibulin 1 (FBLN1) ELISA Kit | MyBioSource MBS2023994 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fibulin 1 (FBLN1) ELISA Kit

catalog :

MBS2023994

quantity :

24-Strip-Wells

price :

270 USD

product information

catalog number :

MBS2023994

products type :

ELISA Kit

products full name :

Fibulin 1 (FBLN1) ELISA Kit

products short name :

[Fibulin 1 (FBLN1)]

other names :

[Fibulin 1; Fibulin-1; fibulin-1; fibulin 1]

products gene name :

[FBLN1]

other gene names :

[FBLN1; FBLN1; FBLN; FIBL1; FIBL-1]

uniprot entry name :

FBLN1_HUMAN

reactivity :

Human

sequence length :

683

specificity :

This assay has high sensitivity and excellent specificity for detection of FAPa. No significant cross-reactivity or interference between FAPa and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.31ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FAPa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FAPa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Tumor immunity

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FAPa in human serum, plasma, tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FAPa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FAPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FAPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FAPa in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

18490682

ncbi acc num :

AAH22497.1

uniprot acc num :

P23142

ncbi mol weight :

74,434 Da

ncbi pathways :

Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Molecules Associated With Elastic Fibres Pathway (730311)

ncbi summary :

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]

uniprot summary :

FBLN1: Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation. Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane. Molecular Function: protease activator activity; extracellular matrix structural constituent; calcium ion binding. Biological Process: extracellular matrix organization and biogenesis; viral reproduction; embryo implantation. Disease: Synpolydactyly 2

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

665

size4 :

5x96-Strip-Wells

price4 :

2715

size5 :

10x96-Strip-Wells

price5 :

4895