ELISA/assay

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit

MBS2023969

48-Strip-Wells

525 USD

ELISA Kit

Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit

Vascular Endothelial Growth Factor C (VEGFC)

VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein

vascular endothelial growth factor C preproprotein; Vascular endothelial growth factor C; vascular endothelial growth factor C; vascular endothelial growth factor C; Flt4 ligand; Flt4-L; Vascular endothelial growth factor-related protein; VRP

VEGFC

VEGFC; VEGFC; VRP; Flt4-L; LMPH1D; VEGF-C; Flt4-L; VRP

VEGFC_HUMAN

Mouse

419

This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor C (VEGFC). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor C (VEGFC) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.9pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor C (VEGFC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor C (VEGFC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor C (VEGFC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor C (VEGFC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor C (VEGFC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Vascular Endothelial Growth Factor C (VEGFC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4885653

NP_005420.1

NM_005429.4

P49767

46,883 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Heart Development Pathway (198802); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105)

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]

VEGFC: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Homodimer; non-covalent and antiparallel. Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. Belongs to the PDGF/VEGF growth factor family. Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q34.3. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: protein binding; growth factor activity; vascular endothelial growth factor receptor 3 binding; chemoattractant activity. Biological Process: signal transduction; induction of positive chemotaxis; negative regulation of cell proliferation; morphogenesis of embryonic epithelium; platelet degranulation; positive chemotaxis; regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of cell proliferation; negative regulation of blood pressure; angiogenesis; positive regulation of cell-matrix adhesion; substrate-bound cell migration; response to drug; platelet activation; positive regulation of neuroblast proliferation; positive regulation of blood vessel endothelial cell migration; positive regulation of protein amino acid autophosphorylation; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of protein secretion; positive regulation of cell division; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of epithelial cell proliferation. Disease: Lymphedema, Hereditary, Id

48-Strip-Wells

525 USD

96-Strip-Wells

715

5x96-Strip-Wells

2760

10x96-Strip-Wells

4985