Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit | MyBioSource MBS2023851 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit

catalog :

MBS2023851

quantity :

48-Strip-Wells

price :

605 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2023851

products type :

ELISA Kit

products full name :

Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit

products short name :

Regenerating Islet Derived Protein 3 Alpha (REG3a)

products name syn :

HIP; INGAP; PAP-H; PAP1; PBCGF; REG-III; REG3; Pancreatitis-Associated Protein; Hepatointestinal pancreatic protein; Human proislet peptide; Pancreatitis-associated protein 1

other names :

regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein 3-alpha; regenerating islet-derived protein 3-alpha; regenerating islet-derived 3 alpha; Hepatointestinal pancreatic protein; HIP/PAP

products gene name :

REG3a

other gene names :

REG3A; REG3A; HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III; HIP; PAP; PAP1; REG-3-alpha; HIP/PAP; Reg III-alpha

uniprot entry name :

REG3A_HUMAN

reactivity :

Rat

sequence length :

175

specificity :

This assay has high sensitivity and excellent specificity for detection of REG3a. No significant cross-reactivity or interference between REG3a and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Rat serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.312-20ng/mL. The standard curve concentrations used for the ELISA's were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL. Sensitivity: 0.133ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level REG3a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level REG3a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of REG3a in rat serum, plasma and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG3a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to REG3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of REG3a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4505605

ncbi acc num :

NP_002571.1

ncbi gb acc num :

NM_002580.2

uniprot acc num :

Q06141

ncbi mol weight :

19,395 Da

ncbi summary :

This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2014]

uniprot summary :

REG3A: Might be a stress protein involved in the control of bacterial proliferation. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: extracellular space; cytoplasm. Molecular Function: carbohydrate binding. Biological Process: heterophilic cell adhesion; cell proliferation; multicellular organismal development; negative regulation of keratinocyte differentiation; acute-phase response

size1 :

48-Strip-Wells

price1 :

605 USD

size2 :

96-Strip-Wells

price2 :

820

size3 :

5x96-Strip-Wells

price3 :

3220

size4 :

10x96-Strip-Wells

price4 :

5795

more info or order :

MyBioSource product webpage