ELISA/assay

Copeptin (CPP) ELISA Kit

MBS2023840

24-Strip-Wells

265 USD

ELISA Kit

Copeptin (CPP) ELISA Kit

[Copeptin (CPP)]

[C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin]

[copeptin]

[CPP]

Human

38

This assay has high sensitivity and excellent specificity for detection of copeptin. No significant cross-reactivity or interference between copeptin and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between copeptin and all the analogues, therefore, cross reaction may still exist.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1,000pg/mL. Sensitivity: 6.1pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level copeptin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level copeptin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of copeptin in human serum, plasma and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to copeptin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to copeptin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain copeptin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of copeptin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

225612

24-Strip-Wells

265 USD

48-Strip-Wells

485

96-Strip-Wells

650

5x96-Strip-Wells

2645

10x96-Strip-Wells

4770