ELISA/assay

Interleukin 35 (IL35) ELISA Kit

MBS2023825

24-Strip-Wells

270 USD

ELISA Kit

Interleukin 35 (IL35) ELISA Kit

[Interleukin 35 (IL35)]

[IL35]

Human

This assay has high sensitivity and excellent specificity for detection of IL35. No significant cross-reactivity or interference between IL35 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100pg/mL. Sensitivity: <0.61pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL35 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL35 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Cytokine; Tumor immunity; Infection immunity

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL35 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to IL12A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated monoclonal antibody specific to IL27B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895