Interferon Beta (IFNb) ELISA Kit | MyBioSource MBS2023816 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Interferon Beta (IFNb) ELISA Kit

catalog :

MBS2023816

quantity :

24-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2023816

products type :

ELISA Kit

products full name :

Interferon Beta (IFNb) ELISA Kit

products short name :

[Interferon Beta (IFNb)]

products name syn :

[IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast]

other names :

[interferon beta; Interferon beta; interferon beta; interferon, beta 1, fibroblast; Fibroblast interferon]

products gene name :

[IFNb]

other gene names :

[IFNB1; IFNB1; IFB; IFF; IFNB; IFB; IFNB; IFN-beta]

uniprot entry name :

IFNB_HUMAN

reactivity :

Mouse

sequence length :

187

specificity :

This assay has high sensitivity and excellent specificity for detection of IFNb. No significant cross-reactivity or interference between IFNb and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between IFNb and all the analogues, therefore, cross reaction may still exist.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.8-500pg/mL. Sensitivity: 2.7pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFNb were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFNb were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Cytokine; Tumor immunity; Infection immunity

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFNb in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IFNb. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IFNb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4504603

ncbi acc num :

NP_002167.1

ncbi gb acc num :

NM_002176.2

uniprot acc num :

P01574

ncbi mol weight :

22,294 Da

ncbi pathways :

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833)

uniprot summary :

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to virus; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; cell surface receptor linked signal transduction; negative regulation of viral genome replication; B cell differentiation; positive regulation of innate immune response; defense response to bacterium; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response

size1 :

24-Strip-Wells

price1 :

260 USD

size2 :

48-Strip-Wells

price2 :

465

size3 :

96-Strip-Wells

price3 :

625

size4 :

5x96-Strip-Wells

price4 :

2530

size5 :

10x96-Strip-Wells

price5 :

4565

more info or order :

MyBioSource product webpage