Mannose Binding Lectin (MBL) ELISA Kit | MyBioSource MBS2023812 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mannose Binding Lectin (MBL) ELISA Kit

catalog :

MBS2023812

quantity :

24-Strip-Wells

price :

265 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2023812

products type :

ELISA Kit

products full name :

Mannose Binding Lectin (MBL) ELISA Kit

products short name :

[Mannose Binding Lectin (MBL)]

products name syn :

[MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)]

other names :

[mannose binding lectin, partial; Mannose binding lectin; mannose-binding protein C; mannose-binding lectin (protein C) 2, soluble; Mannose binding lectin]

products gene name :

[MBL]

other gene names :

[MBL2; MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC]

uniprot entry name :

B1PN75_HUMAN

reactivity :

Mouse

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.072ng/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Signal transduction; Infection immunity; Immune molecule

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Binding Lectin (MBL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Binding Lectin (MBL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Binding Lectin (MBL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mannose Binding Lectin (MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

169335948

ncbi acc num :

ACA48258.2

ncbi mol weight :

3,557 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1269241); Creation Of C4 And C2 Activators Pathway (1269243); Immune System Pathway (1269170); Initial Triggering Of Complement Pathway (1269242); Innate Immune System Pathway (1269203); Lectin Pathway Of Complement Activation (1269244); Phagosome Pathway (153910); Phagosome Pathway (153859)

ncbi summary :

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

uniprot summary :

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: extracellular space; collagen; cell surface; extracellular region. Molecular Function: mannose binding; protein binding; calcium ion binding; calcium-dependent protein binding; receptor binding. Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; positive regulation of phagocytosis; defense response to bacterium; acute-phase response; innate immune response; response to oxidative stress; opsonization; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction; complement activation. Disease: Mannose-binding Protein Deficiency

size1 :

24-Strip-Wells

price1 :

265 USD

size2 :

48-Strip-Wells

price2 :

490

size3 :

96-Strip-Wells

price3 :

655

size4 :

5x96-Strip-Wells

price4 :

2660

size5 :

10x96-Strip-Wells

price5 :

4795

more info or order :

MyBioSource product webpage