Glial Fibrillary Acidic Protein (GFAP) ELISA Kit | MyBioSource MBS2023807 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

catalog :

MBS2023807

quantity :

24-Strip-Wells

price :

255 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2023807

products type :

ELISA Kit

products full name :

Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

products short name :

[Glial Fibrillary Acidic Protein (GFAP)]

products name syn :

[Intermediate Filament Protein]

other names :

[glial fibrillary acidic protein; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein]

products gene name :

[GFAP]

other gene names :

[GFAP; GFAP; ALXDRD; GFAP]

uniprot entry name :

GFAP_HUMAN

reactivity :

Human

sequence length :

431

specificity :

This assay has high sensitivity and excellent specificity for detection of gelsolin. No significant cross-reactivity or interference between gelsolin and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5-4,000pg/mL. Sensitivity: 28.2pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level gelsolin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level gelsolin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Infection immunity; Neuroscience

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of gelsolin in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to gelsolin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to gelsolin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain gelsolin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of gelsolin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

251802

ncbi acc num :

AAB22581.1

uniprot acc num :

P14136

ncbi mol weight :

49,508 Da

ncbi pathways :

Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (530744); Signal Transduction Pathway (477114); Signaling By ERBB4 Pathway (530741); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

uniprot summary :

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: membrane; cytoplasm; intermediate filament; cytosol. Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding. Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; intermediate filament organization; neurite regeneration; astrocyte development. Disease: Alexander Disease

size1 :

24-Strip-Wells

price1 :

255 USD

size2 :

48-Strip-Wells

price2 :

455

size3 :

96-Strip-Wells

price3 :

610

size4 :

5x96-Strip-Wells

price4 :

2470

size5 :

10x96-Strip-Wells

price5 :

4445

more info or order :

MyBioSource product webpage