ELISA/assay

Hyaluronan Binding Protein 2 (HABP2) ELISA Kit

MBS2023806

48-Strip-Wells

565 USD

ELISA Kit

Hyaluronan Binding Protein 2 (HABP2) ELISA Kit

Hyaluronan Binding Protein 2 (HABP2)

FSAP; HABP; HGFAL; PHBP; Plasma Hyaluronan Binding Protein; Factor VII Activating Protein; Factor seven-activating protease; Hepatocyte growth factor activator-like protein

hyaluronan binding protein 2; Hyaluronan-binding protein 2; hyaluronan-binding protein 2; hyaluronan binding protein 2; Factor VII-activating protease; Factor seven-activating protease; FSAP

HABP2

HABP2; HABP2; FSAP; HABP; PHBP; HGFAL; HGFAL; PHBP; FSAP

HABP2_HUMAN

Human

534

This assay has high sensitivity and excellent specificity for detection of Hyaluronan Binding Protein 2 (HABP2). No significant cross-reactivity or interference between Hyaluronan Binding Protein 2 (HABP2) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 6.25-400ng/mL. Sensitivity: Typically less than 2.74ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronan Binding Protein 2 (HABP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronan Binding Protein 2 (HABP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronan Binding Protein 2 (HABP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronan Binding Protein 2 (HABP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronan Binding Protein 2 (HABP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Hyaluronan Binding Protein 2 (HABP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119569890

EAW49505.1

Q14520

59,864 Da

The protein encoded by this gene is an extracellular serine protease that binds hyaluronic acid and is involved in cell adhesion. The encoded protein is synthesized as a single chain, but then undergoes an autoproteolytic event to form the functional heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This protease is known to cleave urinary plasminogen activator, coagulation factor VII, and the alpha and beta chains of fibrinogen, but not prothrombin, plasminogen, or the gamma chain of fibrinogen. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

HABP2: Cleaves the alpha-chain at multiple sites and the beta- chain between Lys-53 and Lys-54 but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro- urokinase) to the active two chain form. Activates coagulation factor VII. Belongs to the peptidase S1 family. Protein type: EC 3.4.21.-; Secreted, signal peptide; Protease; Motility/polarity/chemotaxis; Cell adhesion; Secreted. Chromosomal Location of Human Ortholog: 10q25.3. Cellular Component: extracellular space; extracellular region. Molecular Function: glycosaminoglycan binding; serine-type endopeptidase activity. Biological Process: cell adhesion; proteolysis. Disease: Thrombophilia Due To Thrombin Defect; Thyroid Cancer, Nonmedullary, 5

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340