Inter Alpha-Globulin Inhibitor H5 (ITIH5) ELISA Kit | MyBioSource MBS2023791 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Inter Alpha-Globulin Inhibitor H5 (ITIH5) ELISA Kit

catalog :

MBS2023791

quantity :

24-Strip-Wells

price :

270 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2023791

products type :

ELISA Kit

products full name :

Inter Alpha-Globulin Inhibitor H5 (ITIH5) ELISA Kit

products short name :

[Inter Alpha-Globulin Inhibitor H5 (ITIH5)]

products name syn :

[Inter-alpha-trypsin inhibitor heavy chain H5]

other names :

[inter-alpha-trypsin inhibitor heavy chain H5 isoform 2; Inter-alpha-trypsin inhibitor heavy chain H5; inter-alpha-trypsin inhibitor heavy chain H5; inter-alpha-trypsin inhibitor heavy chain family, member 5]

products gene name :

[ITIH5]

other gene names :

[ITIH5; ITIH5; ITI-HC5; PP14776; KIAA1953; ITI heavy chain H5; ITI-HC5; Inter-alpha-inhibitor heavy chain 5]

uniprot entry name :

ITIH5_HUMAN

reactivity :

Human

sequence length :

728

specificity :

This assay has high sensitivity and excellent specificity for detection of Inter Alpha-Globulin Inhibitor H5 (ITIH5). No significant cross-reactivity or interference between Inter Alpha-Globulin Inhibitor H5 (ITIH5) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 3.12-200ng/mL. Sensitivity: < 1.37ng/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inter Alpha-Globulin Inhibitor H5 (ITIH5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inter Alpha-Globulin Inhibitor H5 (ITIH5) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Metabolic pathway

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H5 (ITIH5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H5 (ITIH5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H5 (ITIH5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Inter Alpha-Globulin Inhibitor H5 (ITIH5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

312434033

ncbi acc num :

NP_116206.4

ncbi gb acc num :

NM_032817.5

ncbi mol weight :

27,191 Da

ncbi summary :

This gene encodes a heavy chain component of one of the inter-alpha-trypsin inhibitor (ITI) family members. ITI proteins are involved in extracellular matrix stabilization and in the prevention of tumor metastasis. They are also structurally related plasma serine protease inhibitors and are composed of a light chain and varying numbers of heavy chains. This family member is thought to function as a tumor suppressor in breast and thyroid cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

uniprot summary :

ITIH5: May act as a tumor suppressor. Belongs to the ITIH family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 10p14. Cellular Component: extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: hyaluronan metabolic process

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

665

size4 :

5x96-Strip-Wells

price4 :

2715

size5 :

10x96-Strip-Wells

price5 :

4895

more info or order :

MyBioSource product webpage